Part:BBa_J70447

yfp RBS, {0,5;15,10} family member - level 1000 (reverse oligo)

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

Also gtg starts are removed to make the RBS more predictable.

Form of insert:

{promoter} ta ctaga ^ g {rbs master sequence} a tgagc ^ {rest of YFP (E0030)}

{promoter} at ^ gatct c {rbs master sequence} t^ actcg {rest of YFP (E0030)}

Forward oligo:

g {rbs master sequence} a tgagc

Reverse oligo:

Reverse complement of (ctagag {rbs master sequence} a)

Usage and Biology

yfp RBS, {0,5;15,10} family member - level 10 (calculated 9.8)
Master sequence=BBa_J70439, forward oligo=BBa_J70440, or reverse oligo=BBa_J70441
yfp RBS, {0,5;15,10} family member - level 100 (calculated 110.5)
Master sequence=BBa_J70442, forward oligo=BBa_J70443, or reverse oligo=BBa_J70444
yfp RBS, {0,5;15,10} family member - level 1000 (calculated 1051.4)
Master sequence=BBa_J70445, forward oligo BBa_J70446, or reverse oligo BBa_J70447
yfp RBS, {0,5;15,10} family member - level 10000 (calculated 9096.9)
Master sequence=BBa_J70448, forward oligo=BBa_J70449, or reverse oligo=BBa_J70450
yfp RBS, {0,5;15,10} family member - level 100000 (calculated 107160.2)
Master sequence=BBa_J70451, forward oligo=BBa_J70452, or reverse oligo=BBa_J70453
yfp RBS, {0,5;15,10} family member - B0029 simulator (calculated 27.8)
Master sequence=BBa_B0029, forward oligo=BBa_J70454, or reverse oligo=BBa_J70455
yfp RBS, {0,5;15,10} family member - B0030 simulator (calculated 413.8)
Master sequence=BBa_B0030, forward oligo=BBa_J70456, or reverse oligo=BBa_J70457
yfp RBS, {0,5;15,10} family member - B0031 simulator (calculated 38.2)
Master sequence=BBa_B0031, forward oligo=BBa_J70458, or reverse oligo=BBa_J70459
yfp RBS, {0,5;15,10} family member - B0032 simulator (calculated 67.9)
Master sequence=BBa_B0032, forward oligo=BBa_J70460, or reverse oligo=BBa_J70461
yfp RBS, {0,5;15,10} family member - B0033 simulator (calculated 4.1)
Master sequence=BBa_B0033, forward oligo=BBa_J70462, or reverse oligo=BBa_J70463
yfp RBS, {0,5;15,10} family member - B0034 simulator (calculated 378)
Master sequence=BBa_B0034, forward oligo=BBa_J70464, or reverse oligo=BBa_J70465
yfp RBS, {0,5;15,10} family member - B0035 simulator (calculated 470)
Master sequence=BBa_B0035, forward oligo=BBa_J70466, or reverse oligo=BBa_J70467

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]